Normalization of mass spectrometry data (NOMAD)

cDNA Microarray Data Normalization

MetTailor: dynamic block summary and intensity normalization for robust analysis of mass spectrometry data in metabolomics

MOTIVATION Accurate cross-sample peak alignment and reliable intensity normalization is a critical step for robust quantitative analysis in untargetted metabolomics since tandem mass spectrometry (MS/MS) is rarely used for compound identification. Therefore shortcomings in the data processing steps can easily introduce false positives due to misalignments and erroneous normalization adjustments...

Normalization, Baseline Correction and Alignment of High-throughput Mass Spectrometry Data

We propose several preprocessing steps to be used before biomarker clustering or classifying for high-throughput Mass Spectrometry (MS) data. These preprocessing steps for the mass spectra are multiple alignment of technical replicates, baseline correction and normalization along the mass/charge axis. While the benefits from baseline correction and alignment seem obvious we studied more careful...

Normalization Regarding Non-Random Missing Values in High-Throughput Mass Spectrometry Data

We propose a two-step normalization procedure for high-throughput mass spectrometry (MS) data, which is a necessary step in biomarker clustering or classification. First, a global normalization step is used to remove sources of systematic variation between MS profiles due to, for instance, varying amounts of sample degradation over time. A probability model is then used to investigate the inten...

Improving the normalization of complex interventions: measure development based on normalization process theory (NoMAD): study protocol

BACKGROUND Understanding implementation processes is key to ensuring that complex interventions in healthcare are taken up in practice and thus maximize intended benefits for service provision and (ultimately) care to patients. Normalization Process Theory (NPT) provides a framework for understanding how a new intervention becomes part of normal practice. This study aims to develop and validate...